Anti-alpha Tubulin Antibody, tyrosinated

半岛bd体育手机客户端 说明

一般描述

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

特异性

This antibody detects the tyrosinated form of alpha Tubulin.

免疫原

KLH-conjugated linear peptide corresponding to human tyrosinated alpha Tubulin.

应用

Western Blotting Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin using purified porcine tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

ELISA Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin, in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected tyrosinated alpha Tubulin and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, tyrosinated validated for use in western blotting, ELISA & ICC.

质量

Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, tyrosinated in 10 ?g of untreated NIH/3T3 cell lysate and demonstrated a loss of signal in PCA treated NIH/3T3 cell lysate.

目标描述

~51 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Unpurified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	mouse, monkey
species reactivity (predicted by homology)	rat (based on 100% sequence homology), human (based on 100% sequence homology)
technique(s)	ELISA: suitable immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_001257328
UniProt登记号	Q71U36
运输	wet ice
Gene Information	human ... TUBA1A(7846)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1