半岛bd体育手机客户端 说明
一般描述
Aiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation.
免疫原
Recombinant protein corresponding to human Aiolos.
应用
Research Sub Category
Transcription Factors
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).
Western Blotting Analysis: A 1 ?g/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate
This Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos.
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 ?g/mL dilution of this antibody detected Aliolos in 10 ?g of a Jurkat cell lysate.
目标描述
~ 58-70 kDa observed
外形
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 9D10, monoclonal |
species reactivity | human, mouse |
technique(s) | ChIP: suitable (ChIP-seq) western blot: suitable |
同位素/亚型 | IgG2bκ |
NCBI登记号 | NP_001244337 |
UniProt登记号 | Q9UKT9 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich