Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28

半岛bd体育手机客户端 说明

一般描述

Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.

特异性

Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.

免疫原

FFGVGGEEDC-KLH peptide
Epitope: N-terminus FFGVG neoepitopes

应用

Research Category
Cell Structure
Western blot

ELISA

Optimal working dilutions must be determined by the end user.
Research Sub Category
ECM Proteins

Inflammation & Autoimmune Mechanisms
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.

外形

Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Format: Purified
Protein A purified

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Cartilage, neural tube, and brain tissue

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	AF-28, monoclonal
species reactivity	bovine, human, rat, mouse, pig
should not react with	guinea pig, horse
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_001135.2 NM_013227.2
UniProt登记号	P16112
运输	wet ice
Gene Information	human ... ACAN(176)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable