Anti-Acylation Stimulating Protein Antibody, clone 4H3

半岛bd体育手机客户端 说明

一般描述

The protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

免疫原

linear peptide corresponding to Human Acylation Stimulating Protein.

应用

ELISA Analysis: 7 ?g/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)
Research Sub Category
Insulin/Energy Signaling

Lipid Metabolism & Weight Regulation
Research Category
Signaling

Metabolism
This Anti-Acylation Stimulating Protein Antibody, clone 4H3 is validated for use in Western Blotting and ELISA for the detection of Acylation Stimulating Protein.

质量

Evaluated by Western Blotting on purified Acylation Stimulating Protein from transfected HEK293 cell lysate

Western Blotting Analysis: 0.1 ?g/mL of this antibody detected purified Acylation Stimulating Protein from transfected HEK293 cell.

目标描述

~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	4H3, monoclonal
species reactivity	human
technique(s)	ELISA: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000055
UniProt登记号	P01024
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable