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位置: 首页> 品牌> Sigma-Aldrich> Anti-ACLY, clone 5F8D11 Antibody

Anti-ACLY, clone 5F8D11 Antibody

品牌
Sigma-Aldrich
货号
MABC697
规格纯度
clone 5F8D11, from mouse
价格
5854.74 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-ACLY, clone 5F8D11 Antibody
ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.

免疫原

Purified recombinant fragment of human ACLY expressed in E. Coli

应用

This Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.
Research Sub Category
Apoptosis - Additional
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ACLY in esophageal cancer tissue.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ACLY in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ACLY in HeLa cells.

ELISA: A 1:10,000 dilution from a representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ACLY in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

目标描述

~125 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Purified
Purified mouse monoclonal in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 5F8D11, monoclonal
species reactivity mouse, monkey, human
technique(s) ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型 IgG1
UniProt登记号 P53396
运输 wet ice
Gene Information human ... ACLY(47)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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