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位置: 首页> 品牌> Sigma-Aldrich> Anti-acetyl-Histone H2B (Lys24) Antibody

Anti-acetyl-Histone H2B (Lys24) Antibody

品牌
Sigma-Aldrich
货号
ABE269
规格纯度
from rabbit, purified by affinity chromatography
价格
6694.05 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-acetyl-Histone H2B (Lys24) Antibody
Histone H2B type 3-B, H2B type 12
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

特异性

This antibody recognizes Histone H2B acetylated at Lys24.

免疫原

Epitope: Acetylated Lys24
KLH-conjugated linear peptide corresponding to human Histone H2B acetylated at Lys24.

应用

Immunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated acetyl-Histone H2B (Lys24) from HeLa acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-acetyl-Histone H2B (Lys24) (1:500 dilution).
Use Anti-acetyl-Histone H2B (Lys24) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP, DB to detect acetyl-Histone H2B (Lys24) also known as H2BK24Ac, Histone H2B (acetyl K24).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blot in recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts.

Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H2B on 10 ?g of recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts.

目标描述

~16 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 300
生物来源 rabbit
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human, rat, mouse
technique(s) dot blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号 NP_001019770
UniProt登记号 Q8N257
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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