Anti-Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2

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一般描述

Histone H3.1t (UniProt: Q16695; also known as H3K27Ac, Histone H3 (acetyl K27), H3 histone family member T, histone cluster 3, H3t) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone acetylation and deacetylation are reversible and play a major role in determining chromatin structure and function. Histone acetylation occurs mainly on the amino-terminal tail domains of histones. Acetylation neutralizes positive charges on histone and promotes the less condensed and more accessible chromatin conformation known as euchromatin. Histone acetylation is vital in regulating transcriptional activation and DNA replication, recombination, and repair. Histone H3 is one of the five ain histone proteins involved in the structure of chromatin in eukaryotic cells. It is the most extensively modified of the five histones. It features a main globular domain and a long N-terminal tail. Histone H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

特异性

Clone 5E2.2 detects histone H3 acetylated on lysine27 in human.

免疫原

KLH-cojugated peptide corresponding to a sequence around lysine 27 of yeast histone H3.

应用

Acetyl Histone H3 (Lys27) Antibody, clone 5E2.2, Cat. No. MABE670, is a highly specific mouse monoclonal antibody, that targets Acetyl Histone H3 (Lys27) and has been tested in Western Blotting and Immunocytochemistry and Dot Blot.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in Hela cells.



Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl Histone H3 (Lys27) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

质量

Evaluated by Western Blotting in HeLa cells stimulated with Sodium Butyrate.


Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Acetyl Histone H3 (Lys27) in 10 ?g of HeLa cells stimulated with Sodium Butyrate.

目标描述

~17 kDa observed; 15.5 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 05-1334

外形

Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	5E2.2, monoclonal
species reactivity	human
technique(s)	dot blot: suitable immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_009564
UniProt登记号	P61830
运输	ambient

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable