Anti-a-Synuclein, clone 10D2 Antibody

半岛bd体育手机客户端 说明

一般描述

α-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing.
This antibody can be used as a sandwich ELISA pair with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4.

免疫原

Recombinant protein corresponding to human Synuclein.

应用

Anti-a-Synuclein, clone 10D2 Antibody is an antibody against a-Synuclein for use in western blotting, IHC & ELISA.
Immunohistochemistry Analysis: A representative lot detected aggregated a-Synuclein in human brain tissues.

ELISA Analysis: A representative lot from an independent laboratory detected aggregated a-Synuclein in a sandwich ELISA with Cat. No. MABN389, Anti-Aggregated a-Synuclein, clone 5G4 (Kovacs, G. G., et al. (2012). Acta Neuropathol. 123(1):37-50.).

质量

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.5 ?g/mL of this antibody detected a-Synuclein in 10 ?g of human brain tissue lysate.

目标描述

~15 kDa observed

联系

Replaces: 04-1053

外形

Affinity purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	10D2, monoclonal
纯化方式	affinity chromatography
species reactivity	human
technique(s)	ELISA: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000336
UniProt登记号	P37840
运输	wet ice
Gene Information	human ... SNCA(6622)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable