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Anti-αActinin antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200813
规格纯度
clone BM-75.2, purified from hybridoma cell culture
价格
2067.58 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-α Actinin antibody, Mouse monoclonal
Alpha-actinin cytoskeletal isoform
F-actin cross-linking protein
Non-muscle alpha-actinin-1
Alpha-actinin-1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti- α Actinin (mouse IgM isotype) is derived from the hybridoma BM-72.5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells. α-Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle, α-actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle, α-actinin has been detected in dense bodies and plaques characteristic of the tissue.

特异性

Monoclonal Anti-α-Actinin specifically recognizes α-Actinin from human1, mouse2, rat3, chicken4, monkey, canine and bovine origin.

免疫原

Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.

应用

Anti-α Actinin antibody may be used in various immunochemical techniques including Immunoblot (~100 kDa), Immunohistochemistry and Immunofluorescence. Monoclonal Anti-α-Actinin may be used for immunofluorescent localization of α-actinin in cultured cells and tissues, for immunofluorescent labeling of skeletal muscle (normal and pathological) in order to detect its organizational state, studies of membrane anchorage sites and immunochemical identification of α-actinin by immunoblotting analysis.

生化/生理作用

α-actinin has extensive proteins association with actin containing stress fibers, in particular with their membrane bound termini.
α-actinin modulates muscle contraction and has a role in cytoskeletal organisation. α-actinin acts as an actin crosslinker and scaffold. Mutations in this gene has been linked to heterogeneous hypertrophic cardiomyopathy and juvenile onset atrial fibrillation.

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoor in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 mouse
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 BM-75.2, monoclonal
分子量 ~100 kDa
species reactivity human
包装 antibody small pack of 25 μL
浓度 ~1 mg/mL
technique(s) immunoblotting: 0.5-1 μg/mL using human HeLa cells extract.
immunofluorescence: 5-10 μg/mL using human foreskin fibroblast Hs68 cells.
同位素/亚型 IgM
UniProt登记号 P12814
运输 dry ice
储存温度 ?20℃
Gene Information human ... ACTN1(87)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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