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位置:首页 > 品牌 > Sigma-Aldrich > Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31

品牌
Sigma-Aldrich
货号
MABE251
规格纯度
clone HMC 31, from mouse
价格
6022.61 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

特异性

This antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.

免疫原

BSA-conjugated 5-hydroxymethylcytosine.
Epitope: 5-hydroxymethylcytosine

应用

Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
ELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.

Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.

质量

Evaluated by Dot Blot in unmodified and modified cytosine DNA.

Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.

目标描述

This antibody detects 5-hydroxymethylcytosine.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Unmodified and modified cytosine DNA.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

半岛bd体育手机客户端 性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆HMC 31, monoclonal
species reactivity (predicted by homology)all
technique(s)ELISA: suitable
dot blot: suitable
methylated DNA immunoprecipitation (MeDIP): suitable
同位素/亚型IgG1κ
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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