半岛bd体育手机客户端 说明

一般描述

Amicon^?Ultra-2 mL centrifugal devices with Ultracel^?100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at 95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon^?Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^?Ultra 2 mL filters deliver the highest recovery with shortest spin times.

应用

• Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel^?membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates.
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

特点和优势

Minimum Final Concentrate Volume: 15 ?L–70 ?L

联系

Replaces: 4211

外形

Color Code: 透明

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

eCl@ss	36100101
NACRES	NB.24

半岛bd体育手机客户端 性质

质量水平	400
物料	polypropylene tube (for collection) regenerated cellulose membrane styrene-butadiene housing
无菌性	non-sterile
半岛bd体育手机客户端 线	Amicon?
manufacturer/tradename	Amicon?Ultra
technique(s)	protein extraction: suitable protein purification: suitable
长度	119.7 mm
直径	15.9 mm
过滤面积	1 cm2
孔径	100 kDa NMWCO
试验参数	sample volume: 2 mL
运输	ambient