半岛bd体育手机客户端 说明

一般描述

Amicon^?Ultra-2 mL centrifugal devices with Ultracel^?3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at 100% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon^?Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^?Ultra 2 mL filters deliver the highest recovery with shortest spin times.

应用

• Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel^?membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates.
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

特点和优势

Minimum Final Concentrate Volume: 15 ?L–70 ?L

联系

Replaces: 4202

外形

Color Code: 透明

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

eCl@ss	36100101
NACRES	NB.24

半岛bd体育手机客户端 性质

质量水平	400
物料	polypropylene tube (for collection) regenerated cellulose membrane styrene-butadiene housing
无菌性	non-sterile
半岛bd体育手机客户端 线	Amicon?
manufacturer/tradename	Amicon?Ultra
technique(s)	protein extraction: suitable protein purification: suitable
长度	119.7 mm
直径	15.9 mm
过滤面积	1 cm2
孔径	3 kDa NMWCO
试验参数	sample volume: 2 mL
运输	ambient