半岛bd体育手机客户端 说明
一般描述
Alpha-Galactosidase (α-Gal) hydrolyzes alpha-galactosyl moieties found in glycolipids and glycoproteins. In mammals, a-Gal hydrolyzes poly- and oligosaccharides commonly found in dietary sources that are difficult to digest. Therefore, α-Gal is used in dietary supplements that help to reduce the production of intestinal gases due to consumption of certain foods. It is known total α-Gal activity is due to two major isozymes with unique, yet different thermostability profiles. Alpha-Galactosidase A, is thermolabile and represents approximately 90% of total α-Gal activity. Fabry Disease, a lysosomal disease disorder, is characterized by mutations in alpha-Galactosidase A. These mutations cause abnormal accumulation of glycosphingolipids in lysosomes.
特点和优势
The Alpha Galactosidase (α-Gal) Activity Assay Kit:
适用性
The kit is suitable for the detection of α-Galactosidase activity in tissue homogenates (kidney, etc.), cell lysates (U937, etc.) and biological fluids (saliva, etc.).
原理
The Alpha Galactosidase (α-Gal) Activity Assay Kit provides a simple, rapid way to monitor total α-Gal activity in a wide variety of biological samples. In this kit, α-Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (λex= 360 nm/λem= 445 nm). The assay is specific (beta galactosidase activity is not detected), sensitive and can detect as low as 0.1 μU of α-Galactosidase activity.
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| web metadata keyword.default | Alpha Galactosidase (a-Gal) Activity Assay Kit (Fluorometric)|sigma-aldrich |
| detection method | fluorometric |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich
m.cnreagent.com
