ALK2 (R206H), Active

半岛bd体育手机客户端 说明

应用

Useful for kinase assay.

生化/生理作用

Recombinant human ALK2 (R206H) (147-end) was expressed byBaculovirusin Sf9 insect cells using an N-terminal GST tag. ALK2 belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins which encodes activin A type I receptor. ALK2 signals a particular transcriptional response in concert with activin type II receptors. Mutations in ALK2 are associated with fibrodysplasia ossificans progressiva (FOP) and the mutations are located in or adjacent to the GS regulatory region or active site of the kinase. The mutations in ALK2 were predicted by protein structure homology modeling to activate the ALK2 protein and enhance receptor signaling⁽¹⁾. Dysregulated ALK2 kinase activity plays a role in the pathogenesis of FOP⁽²⁾.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
测定	70-95% (SDS-PAGE)
形式	frozen liquid
specific activity	5-7 nmol/min·mg (radiometric)
分子量	~67 kDa
浓度	0.1 μg/μL
登记号	NM_001105
运输	dry ice
储存温度	?70℃
Gene Information	human ... ALK2(90)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable