半岛bd体育手机客户端 说明
应用
Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.
Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
所有缓冲液的pH值都与温度和浓度有关。对于Tris缓冲液,降温每℃其pH值约增加0.03单位,每10倍稀释其pH降低0.03-0.05单位。
对于精确的应用,请使用带有玻璃/甘汞组合电极并仔细校准过的pH计。
法律信息
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of sigma-aldrich Co. LLC
基本信息
线性分子式 | NH2C(CH2OH)3· HCl |
分子量 | 157.60 |
Beilstein | 3675235 |
MDL编号 | MFCD00012590 |
半岛bd体育手机客户端 性质
质量水平 | 400 |
生物来源 | synthetic |
描述 | Manufactured under appropriate controls for use as a raw material in BioProcessing |
半岛bd体育手机客户端 线 | EMPROVE?EVOLVE |
形式 | powder |
technique(s) | cell culture | mammalian: suitable |
杂质 | Endotoxin, tested Microbial, tested Trace Metals, tested |
useful pH range | 7.0-9.0 |
pKa (25 ℃) | 8.1 |
适用性 | suitable for manufacturing use |
异质活性 | Cytotoxicity, contains (tested) DNase, contains (tested) NICKase, contains (tested) Protease, contains (tested) RNase, contains (tested) |
SMILES string | Cl.NC(CO)(CO)CO |
InChI | 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H |
InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich