Thrombomodulin human

半岛bd体育手机客户端 说明

一般描述

Thrombomodulin is an integral membrane protein that spans a length of 559 residues and contains five structural domains. The hydrophobic amino terminal domain is made up of 223 amino acid residues. The adjacent domain contains six EGF type B repeats spanning a length of 236 residues. This is followed by a 34 amino acid-long Ser/Thr-rich domain, which may be involved in 0-linked glycosylation. The next domain is a putative membrane-spanning domain of 23 residues, followed by a carboxyl-terminal cytosolic tail of 38 residues. The gene encoding this protein lacks introns. Recombinant soluble thrombomodulin is a 491 amino acid glycoprotein containing the extracellular domain of thrombomodulin.

应用

Thrombomodulin human has been used to bind to anti-TM for peptide cleavage.

生化/生理作用

Thrombomodulin (TM) is an endothelial cell expressed transmembrane glycoprotein that can form a complex with the coagulation factor, thrombin. The thrombomodulin/thrombin complex converts protein C to its activated form, protein Ca, which in turn proteolytically cleaves and deactivates factor Va and factor VIIIa, two essential components of the coagulation mechanism. This inactivation reduces the generation of additional thrombin and thereby effectively prevents continued coagulation. Reduced levels of thrombomodulin can correlate with the pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. However, the serum levels of the truncated circulating form of thrombomodulin are typically elevated during inflammation and in the presence of various inflammatory related diseases.

序列

APAEPQPGGS QCVEHDCFAL YPGPATFLNA SQICDGLRGH LMTVRSSVAA DVISLLLNGD GGVGRRRLWI GLQLPPGCGD PKRLGPLRGF QWVTGDNNTS YSRWARLDLN GAPLCGPLCV AVSAAEATVP SEPIWEEQQC EVKADGFLCE FHFPATCRPL AVEPGAAAAA VSITYGTPFA ARGADFQALP VGSSAAVAPL GLQLMCTAPP GAVQGHWARE APGAWDCSVE NGGCEHACNA IPGAPRCQCP AGAALQADGR SCTASATQSC NDLCEHFCVP NPDQPGSYSC MCETGYRLAA DQHRCEDVDD CILEPSPCPQ RCVNTQGGFE CHCYPNYDLV DGECVEPVDP CFRANCEYQC QPLNQTSYLC VCAEGFAPIP HEPHRCQMFC NQTACPADCD PNTQASCECP EGYILDDGFI CTDIDECENG GFCSGVCHNL PGTFECICGP DSALARHIGT DCDSGKVDGG DSGSGEPPPS PTPGSTLTPP A

外形

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

重悬

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in HEK 293 cells
测定	≥98% (HPLC) ≥98% (SDS-PAGE)
形式	lyophilized
分子量	51.4 kDa
包装	pkg of 10 μg
technique(s)	cell culture | mammalian: suitable
杂质	<0.1 EU/μg endotoxin, tested
颜色	white
UniProt登记号	P07204
运输	wet ice
储存温度	?20℃
Gene Information	human ... THBD(7056)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable