MMP-3 human

半岛bd体育手机客户端 说明

一般描述

The gene JE is an early growth response gene that was first identified in mouse 3T3 cells. The gene is mapped to mouse chromosome 11. It encodes a member of the CC subfamily, the members of which contain characteristic two adjacent cysteine residues.
Active MMP-3 catalytic domain from human cDNA, expressed in E. coli and purified using proprietary technologies. The active MMP-3 is very useful in studying enzyme kinetics, cleave target substrates, and screen for inhibitors.

应用

MMP-3 human has been used to develop a simple and sensitive electrogenerated chemiluminescence (electrochemiluminescence (ECL)) peptide-based bioassay for the detection of matrix metalloproteinases releasing from living cells.
MCP-1 has been used to induce chemotaxis in mice macrophages.

生化/生理作用

The gene JE, also referred to as MCP-1 (monocyte chemoattractant protein-1), encodes a secreted chemokine that functions as a chemoattractant for monocytes and may activate their anti-tumor properties. This protein may serve as a potential therapeutic anticancer agent.
Matrix metallopeptidase 3 (MMP3) has a broad substrate specificity and is capable of degrading fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. It participates in wound repair, progression of atherosclerosis, tissue remodeling and tumor initiation. MMP3 variants can be associated with hypertrophy of interventricular septum or hypertrophic cardiomyopathy.

外形

Lyophilized powder.

重悬

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

基本信息

MDL编号	MFCD00282364
NACRES	NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
测定	≥95% (HPLC) ≥95% (SDS-PAGE)
形式	lyophilized
分子量	~19.5 kDa
包装	pkg of 10 μg
technique(s)	western blot (chemiluminescent): suitable
杂质	endotoxin, tested
NCBI登记号	P08254
运输	wet ice
储存温度	?20℃
Gene Information	human ... MMP3(4314)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable