Immobilon^?-P PVDF Membrane

半岛bd体育手机客户端 说明

一般描述

Primary Binding Mechanisms: 静电，疏水
Filter Code: IPVH
Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 ?m pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore′s Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 ?m pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 ?m pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon^?PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths

应用

10 sheets, 8 cm x 10 cm, 0.45 ?m pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

eCl@ss	32031602
NACRES	NB.22

半岛bd体育手机客户端 性质

物料	PVDF membrane plain filter white filter
质量水平	400
特点	hydrophobic
manufacturer/tradename	Immobilon?
technique(s)	western blot: suitable
过滤器长度 × 宽度	8 cm × 10 cm
孔径	0.45 μm pore size
容量	160 μg/cm2adsorption capacity (insulin) 215 μg/cm2adsorption capacity (BSA) 294 μg/cm2adsorption capacity (goat IgG)
相容性	for use with Amido black for use with CPTS for use with Colloidal gold for use with Coomassie brilliant blue for use with India ink for use with Ponceau-S red for use with Sypro ruby for use with Toluidine blue for use with Transillumination
detection method	chemiluminescent colorimetric fluorometric radioactive
运输	ambient