半岛bd体育手机客户端 说明

一般描述

The enzyme enterokinase is a serine protease that is encoded by a 3696 nucleotide cDNA that contains an open reading frame of 3057 nucleotides. The encoded precursor is cleaved to form an active protein that contains a 784 amino acid heavy chain followed by a 235 amino acid light chain that are linked by one or more disulfide bonds. The mRNA is found to be expressed only in small intestine and the protein is found in enterocytes of duodenum and proximal jejunum . The human enterokinase gene is localized to chromosome 21q21.

应用

Enterokinase has been used in the activation of pancreatic enzyme preparation. It has been used for the activation and determination of trypsin activity in pancreatic enzyme secretion.

生化/生理作用

The enterokinase enzyme is a glycoprotein that catalyzes the conversion of trypsinogen to trypsin, which activates the zymogens pancreatic digestive enzymes. Deficiency of this enzyme causes protein deficiency as zymogens do not get activated. Congenital deficiency of enteropeptidase leads to severe intestinal malabsorption with diarrhea, vomiting, and growth failure.

外形

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 1 mM Calcium Chloride.

重悬

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed in CHO cells
测定	≥90% (SDS-PAGE)
形式	lyophilized powder
分子量	97.5 kDa
包装	pkg of 10 and 50 μg
杂质	<2 EU/μg endotoxin (LAL test)
UniProt登记号	P98073
运输	wet ice
储存温度	?20℃
Gene Information	human ... ENTK(5651)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	nwg