Anti-VEGF Receptor-1 Antibody

半岛bd体育手机客户端 说明

特异性

Recognizes Human Flt-1 (fms-like tyrosine kinase). Flt-1 (human 1338 aa, rat 1336 aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells (Shibuya et al., 1990; Matsushime et al., 1987; Yamane et al., 1994; Finnerty et al., 1993; Choi et al., 1994; Plouet et al., 1989; Simon et al.,

1998; Sait et al., 1995; deVries et al., 1992).

SPECIES REACTIVITIES:

Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.

免疫原

Human recombinant Flt-1 (extracellular domain). Actual epitope recognized by this clone is between 1-250 aa of human Flt-1.

应用

Research Category
Signaling
Anti-VEGF Receptor-1 Antibody is an antibody against VEGF Receptor-1 for use in ELISA & WB.
Western blot: 0.5-2.0 μg/mL. MW = 180 kDa.

Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.

ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.

Optimal working dilutions must be determined by end user.
Research Sub Category
Growth Factors & Receptors

外形

Purified from Ascites. Liquid in PBS and 5% Trehalose.
Format: Purified

储存及稳定性

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze and thaw.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	monoclonal
species reactivity	mouse, human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_002019.3
UniProt登记号	P17948
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable