半岛bd体育手机客户端 说明
一般描述
TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
免疫原
Purified recombinant extracellular fragment of TrkA expressed in HEK293 cells.
应用
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in human brain and rat cerebellum tissues.
Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected TrkA in PC-12 cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-TrkA Antibody, clone 6B2 is a highly specific mouse monoclonal antibody, that targets TrkA & has been tested in western blotting, IHC & ICC.
质量
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TrkA in 10 ?g of human brain tissue lysate.
目标描述
~87 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms ranging between ~77 kDa and ~87 kDa
外形
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Human brain tissue lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 6B2, monoclonal |
species reactivity | rat, human |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NP_002520 |
UniProt登记号 | P04629 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich