Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70

半岛bd体育手机客户端 说明

一般描述

The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

特异性

This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

免疫原

Human embryonal carcinoma cell line 2102Ep

应用

Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70 is an antibody against Stage-Specific Embryonic Antigen-4 for use in FC, ELISA, IF, IH.
Immunohistochemistry: A previous lot of this antibody was used in IH.

Immunofluorescence: A previous lot of this antibody was used in IF.

ELISA: A previous lot of this antibody was used in ELISA.

Flow Cytometry: A starting range of 10-20 ?g/mL is suggested.

Optimal working dilutions must be determined by the end user.

外形

Purified moust monoclonal IgG3 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl, pH 7.6 with 0.1% Sodium Azide
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at from date of receipt.

分析说明

Control
Pluripotent human embryonic stem (ES) cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	MC-813-70, monoclonal
species reactivity	human, mouse
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable flow cytometry: suitable immunofluorescence: suitable immunohistochemistry: suitable
input	sample type: human embryonic stem cell(s) sample type induced pluripotent stem cell(s) sample type: mouse embryonic stem cell(s)
同位素/亚型	IgG3
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2