Anti-p21WAF1 Antibody, clone EA10

半岛bd体育手机客户端 说明

一般描述

The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth.

免疫原

Recombinant protein corresponding to human p21WAF1.

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blot Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).

Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
Anti-p21WAF1 Antibody, clone EA10 is a Mouse Monoclonal Antibody for detection of p21WAF1 & has been validated in WB, FC.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blot in HT-29 cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected p21WAF1 in 10 ?g of HT-29 cell lysate.

目标描述

~21 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HT-29 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	EA10, monoclonal
species reactivity	human
technique(s)	flow cytometry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000380
UniProt登记号	P38936
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable