Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

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一般描述

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

免疫原

Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.

应用

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.

质量

Evaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates.

Western Blot Analysis: 2 ?g/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 ?g of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)

目标描述

~220 kDa observed

外形

Protein G Purified
Format: Purified

分析说明

Control
Untreated and lambda phosphatase treated HeLa cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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