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位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho BAD Antibody (Ser155)

Anti-phospho BAD Antibody (Ser155)

品牌
Sigma-Aldrich
货号
ABC467
规格纯度
from rabbit, purified by affinity chromatography
价格
6022.61 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-phospho BAD Antibody (Ser155)
Bcl2 antagonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-XL/Bcl-2-associated death promoter
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118, Ser-134, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

免疫原

Epitope: BH3 domain
KLH-conjugated linear peptide corresponding to the BH3 domain of human phospho BAD (Ser155).

应用

Research Sub Category
Apoptosis - Additional
Peptide Inhibition Analysis: A 1:100 dilution from a representative lot blocked phospho BAD (Ser155) in Forskolin treated PC12 cell lysates.
Immunoprecipitation Analysis: 5 ?g from a representative lot immunoprecipitated phospho BAD (Ser155) in 0.5 mg of Forskolin treated PC12 cell lysate.
Anti-phospho BAD (Ser155) is an antibody against phospho BAD (Ser155) for use in WB, PIA, IP.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in Forskolin treated PC12 cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho BAD (Ser155) in 10 ?g of Forskolin treated PC12 cell lysate.

目标描述

~20 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
species reactivity (predicted by homology) canine (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s) immunoprecipitation (IP): suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI登记号 NP_031548
UniProt登记号 Q92934
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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