Anti-Pan-Ras Antibody, clone RAS 10

半岛bd体育手机客户端 说明

一般描述

The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.

免疫原

Recombinant protein corresponding to human Pan-Ras.

应用

Western Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
Detect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.

质量

Evaluated by Western Blot in MCF7 cell lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected Pan-Ras in 10 ?g of MCF7 cell lysate.

目标描述

~21 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
MCF7 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	RAS 10, monoclonal
species reactivity	human
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_004976
UniProt登记号	P01116
运输	wet ice
Gene Information	human ... KRAS(3845)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable