Anti-OB-Cadherin Antibody, extracellular domain, clone 16A

半岛bd体育手机客户端 说明

一般描述

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

特异性

Reacts with the extracellular domain of human cadherin-11.

免疫原

Epitope: extracellular domain
Fusion protein corresponding to extracellular domain of human OB-cadherin coupled to GST.

应用

Research Category
Cell Structure
Detect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH.
Western blot: 1:25 - 1:250
Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol.
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells
Optimal working dilutions must be determined by the end user. I/
Research Sub Category
Adhesion (CAMs)

外形

Affinity purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide.
Format: Purified

储存及稳定性

1 year at 4°C from date of shipment

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	16A, monoclonal
species reactivity	rat, human
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_001797.2
UniProt登记号	P55287
运输	wet ice
Gene Information	human ... CDH11(1009)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable