Anti-MDM2 Antibody, clone 4B2C1.11

半岛bd体育手机客户端 说明

一般描述

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

免疫原

Recombinant protein corresponding to human MDM2.

应用

Use Anti-MDM2 Antibody, clone 4B2C1.11 (mouse monoclonal antibody) validated in WB, IP, ICC to detect MDM2 also known as E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cell lysates (Hjerpe, R., et al. (2010). 42(5):725-735.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cells (Hjerpe, R., et al. (2010). 42(5):725-735.).

质量

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.25 ?g/mL of this antibody detected MDM2 in 10 ?g of mouse brain tissue lysate.

目标描述

~55 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	4B2C1.11, monoclonal
species reactivity	mouse, human
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_002383
UniProt登记号	Q00987
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable