Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Anti-Glutathione-S-transferase (GST) antibody, mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein. GST is a Phase II metabolic enzyme, expressed ubiquitously in both eukaryotes and prokaryotes. GSTs are classified in to three types based on their cellular localization, namely cytosolic GSTs, mitochondrial GSTs and microsomal GSTs.

特异性

Monoclonal Anti- Glutathione-S-transferase (GST) recognizes GST from Schistosoma japonicum in GSTfusion proteins.

免疫原

hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein

应用

Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal has been used in immunoblotting.

生化/生理作用

Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore, plays a role in the detoxification of potential alkylating agents.(5) GST protects the cell against oxidative stress and several toxic molecules. It facilitates the synthesis and modification of leukotrienes and prostaglandins. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

储存及稳定性

Store at ?20 ℃. For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze at ?20 ℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02253002
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	2H3-D10, monoclonal
形式	buffered aqueous solution
浓度	~1.0 mg/mL
technique(s)	western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ～ 10ng/lane
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable