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位置: 首页> 品牌> Sigma-Aldrich> Anti-Glial Fibrillary Acidic Protein Antibody

Anti-Glial Fibrillary Acidic Protein Antibody

品牌
Sigma-Aldrich
货号
AB5541
规格纯度
Chemicon?, from chicken
价格
6358.33 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Glial Fibrillary Acidic Protein Antibody
GFAP
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

特异性

Expected to cross-react with bovine and porcine.
Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Other species have not yet been tested.

免疫原

Purified bovine GFAP.

应用

This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.
Immunohistochemistry(paraffin):
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.

Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.

Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.

Optimal working dilutions must be determined by the end user.

STAINING PATTERN:

The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.

质量

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.

目标描述

55 kDa

联系

Replaces: 04-1031; 04-1062

外形

Purified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.
ImmunoAffinity Purified

储存及稳定性

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Astrocytes, astrocytomas, neural stem cells.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 chicken
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human, bovine, pig, mouse, rat
manufacturer/tradename Chemicon?
technique(s) immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI登记号 NM_002055.2
UniProt登记号 P14136
运输 dry ice
Gene Information human ... GFAP(2670)

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2

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