Anti-GLAST Antibody, clone 8C11.1

半岛bd体育手机客户端 说明

一般描述

Excitatory amino acid transporter 1 (UniProt P43003; also known as GLAST-1, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 member 3) is encoded by the SLC1A3 (also known as EA6, EAAT1, GLAST, GLAST1) gene in human (Entrez Gene ID 6507). GLAST-1 is a multitransmembrane protein that functions as a high affinity glutamate transporter and mediates the termination of postsynaptic action of glutamate in central nervous system by rapidly removing released glutamate from the synaptic cleft. GLAST-1 also transports L- and D-aspartate. GLAST gene mutations are linked to episodic ataxia type 6 (EA6), a disorder characterized by seizures, migraine, and alternating hemiplegia. There are 5 known spliced variants. Isoform 2 has no functional glutamate uptake activity and exerts a dominant negative effect against isoform 1.

特异性

Clone 8C11.1 epitope lies within the second extracellular segement of GLAST isoforms 1 & 2. The epitope region is also present in isoform 4, but not in isforms 3 & 5.

免疫原

GST-tagged recombinant protein corresponding to the extracellular domain of human GLAST.
Epitope: Extracellular domain

应用

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLAST in human cerebellum and rat cerebellum tissue.
Anti-GLAST Antibody, clone 8C11.1 is an antibody against GLAST for use in Western Blotting, Immunohistochemistry .
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected GLAST in 200 ?g of human brain tissue lysate.

目标描述

~55 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG1λ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	8C11.1, monoclonal
species reactivity	human, rat
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1λ
NCBI登记号	NP_004163
UniProt登记号	P43003
运输	wet ice
Gene Information	human ... SLC1A3(6507)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable