半岛bd体育手机客户端 说明
一般描述
GATA-1 (GATA1), or alternatively Gata-binding factor 1, GF-1, NF-E1 DNA binding protein, and Erythroid transcription factor (EryF1) encoded by the human gene named GATA1/ERYF1/GF1 is transcriptional activator central for erythroid development. GATA1 also plays critical roles in controlling gene expression in other system as well. Research has shown that GATA1 contributes to the level of alpha synuclein gene expression in Parkinson’s disease and over expression of GATA1 in cortical neurons leads to a decrease in the expression of Rab4b and reduces dendrite branching and maturation, and GATA1 expression is elevated in brains with depressive disorder suggesting that GATA1 may be new target for anti-depressant therapies. EMD-Millipore’s Anti-GATA1 mouse monoclonal antibody has been tested in western blot on K562 cell lysates and paraffin embedded immunohistochemistry on pancreatic cancer tissues and in fluorescent immunocytochemistry on K562 cells in culture.
免疫原
Purified recombinant fragment of human GATA1 expressed in E. Coli.
应用
Anti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC.
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in K562 cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in pancreatic cancer tissue.
Optimal working dilutions must be determined by end user.
质量
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GATA1 in K562 cell lysate.
目标描述
~45 kDa observed. Uncharacterized bands may appear in some lysate(s).
联系
Replaces: MABE470
外形
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
K562 cell lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | 4F5, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2b |
UniProt登记号 | P15976 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich