Anti-Estrogen Receptor Antibody, clone B10

半岛bd体育手机客户端 说明

特异性

The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.

SPECIES REACTIVITIES:

Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.

免疫原

Epitope: a.a. 151-165

应用

Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.
Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000

Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000

ELISA at 1:1,000-1:2,000

Western blot analysis at 1:1,000-1:2,000

Immunoprecipitation at 1:1-1:10

Gel super shifts at 1:1-1:10

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

外形

Ascites. Liquid, does not contain any preservative.

储存及稳定性

Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	B10, monoclonal
species reactivity	human, rat
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG2a
NCBI登记号	NP_000116.2
UniProt登记号	P03372
运输	wet ice
Gene Information	human ... HSPG2(3339)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable