Anti-DNA Antibody, double stranded, clone BV16-13

半岛bd体育手机客户端 说明

特异性

Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

BINDING ASSAY:

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



ANTIGEN BINDING

PROFILE: Relative Binding To:



Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

免疫原

Epitope: double stranded, 28% reactive with single stranded

应用

EIA/RIA

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Apoptosis - Additional

外形

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	culture supernatant
antibody product type	primary antibodies
克隆	BV16-13, monoclonal
species reactivity (predicted by homology)	all
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunocytochemistry: suitable radioimmunoassay: suitable
同位素/亚型	IgG2aκ
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1