Anti-Collagen Type I Antibody

半岛bd体育手机客户端 说明

特异性

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

免疫原

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

应用

Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.

外形

ImmunoAffinity Purified
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose^?4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a registered trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	goat
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	bovine, human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable dot blot: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
适用性	not suitable for immunohistochemistry (Paraffin)
NCBI登记号	NP_000079
UniProt登记号	P02452
运输	wet ice
Gene Information	human ... COL1A1(1277)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable