半岛bd体育手机客户端 说明
一般描述
The antibody detects endogenous levels of total CDK4 protein.
免疫原
Peptide sequence around aa. 295-299 (L-H-K-D-E), according to the protein NP_000066.1
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
特点和优势
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目标描述
Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
外形
Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol
基本信息
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
偶联物 | unconjugated |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
分子量 | ~34 kDa |
species reactivity | human |
浓度 | 1 mg/mL |
technique(s) | western blot: 1:500-1:1000 |
同位素/亚型 | IgG |
免疫原序列 | (L-H-K-D-E) |
NCBI登记号 | NM_000075.2 |
UniProt登记号 | P11802 |
运输 | wet ice |
储存温度 | ?20℃ |
Gene Information | human ... CDK4(1019) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich