Anti-CD54 Antibody, extracellular, clone 84H10

半岛bd体育手机客户端 说明

一般描述

This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1beta or IFN gamma on other cell types such as fibroblasts and enthothelial cells.

特异性

Recognizes the extracellular D1 domain of ICAM-1. Blocks binding of LFA-1 and P. falciparum to ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. Detects an antigen of 90 kDa.

免疫原

K562 cell line
Epitope: extracellular

应用

Research Category
Cell Structure
Detect CD54 using this Anti-CD54 Antibody, extracellular, clone 84H10 validated for use in FC, IP, IH.
Research Sub Category
Adhesion (CAMs)
Flow Cytometry: Neat - 1:10. 10 μL of suggested dilution will label 10e6 cells.

Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections.

Immunoprecipitation: 5 μg/ 10e7 cells

Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1.

Optimal working dilutions must be determined by end user.

外形

Affinity purified immunoglobulin. Liquid in Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide.
Format: Purified

储存及稳定性

Store at 2-8°C for up to 12 months from date of receipt. Alternatively, this antibody can be stored at -20?C. Avoid repeated freeze-thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	84H10, monoclonal
species reactivity	human
should not react with	canine
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable
同位素/亚型	IgG1
适用性	not suitable for immunohistochemistry (Paraffin)
NCBI登记号	NM_000201.1
UniProt登记号	P05362
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable