您好,欢迎来到半岛电竞官方网址 ! [登录] [免费注册]
半岛电竞官方网址
位置:首页 > 品牌 > Sigma-Aldrich > Anti-CD14 Antibody, clone 2D-15C

Anti-CD14 Antibody, clone 2D-15C

品牌
Sigma-Aldrich
货号
MAB1219
规格纯度
clone 2D-15C, Chemicon®, from mouse
价格
5426.21 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
半岛bd体育手机客户端 名称:
Anti-CD14 Antibody, clone 2D-15C
LPS Receptor
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

BIOCHEMISTRY: p I = 5.8

特异性

This antibody belongs to CD14 (assigned by the Third International Workshop on Leucocyte Differentiation Antigens, Oxford, 1986; McMichael et al. Eds., 1986). and reacts with 50 to 55kD protein. It will detect blood monocytes, Kupffer cells, red pulp macrophages, dendritic cells and also "epithelioid" or giant cells within granulomatous tissue (Hancock et al., 1983), foam cells in renal interstitium (Nolasco et al., 1984) and placental dendritic cells and macrophages (Barnett et al., 1984; Brooks et al., 1983; Polli et al., 1984; Hopper et al., 1986). Monocytes react with this antibody in the early stages of differentiation, promonocytes and monoblasts do not. Activity is not lost on monocyte/macrophage activation (Hancock et al., 1983).



Cell reactivity

Reacts with peripheral blood monocytes and weakly with granulocytes. Negative to platelets, erythrocytes and lymphocytes. Sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).

免疫原

Human peripheral blood mononuclear cells.

应用

Anti-CD14 Antibody, clone 2D-15C detects level of CD14 & has been published & validated for use in FC, IH.
Research Sub Category
Immunoglobulins & Immunology
This antibody is an important general cell marker for mononuclear phagocytes in normal samples and a variety of disease states including a proportion of cases of myeloid leukemia. It is suitable for flow cytometry, and immunoperoxidase staining on frozen tissue sections.

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:25



Dilute with isotonic buffer. Use 50 μl per 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.



2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.
Research Category
Inflammation & Immunology

外形

The purified antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Format: Purified

储存及稳定性

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

半岛bd体育手机客户端 性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2D-15C, monoclonal
species reactivityhuman
manufacturer/tradenameChemicon®
technique(s)flow cytometry: suitable
immunohistochemistry: suitable
同位素/亚型IgG1
NCBI登记号NM_000591.2
NM_001040021.1
UniProt登记号P08571
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map