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位置: 首页> 品牌> Sigma-Aldrich> Anti-BRCA1 Antibody, clone MS110

Anti-BRCA1 Antibody, clone MS110

品牌
Sigma-Aldrich
货号
MABC199
规格纯度
clone MS110, from mouse
价格
6358.33 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-BRCA1 Antibody, clone MS110
Breast cancer type 1 susceptibility protein, RING finger protein 53
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.

特异性

This antibody recognizes the N-terminus of BRCA1.

免疫原

Recombinant protein corresponding to the N-terminus of human BRCA1.
Epitope: N-terminus

应用

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected in BRCA1 in MCF7 cells.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).
Anti-BRCA1 Antibody, clone MS110 is an antibody against BRCA1 for use in Western Blotting, ICC.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 ?g/mL of this antibody detected BRCA1 in 10 ?g of HeLa nuclear extract.

目标描述

~220 kDa and ~85 kDa observed. Multiple isoforms exist at various molecular weights and may be subject to post-translational modification.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa nuclear extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 MS110, monoclonal
species reactivity human
technique(s) immunocytochemistry: suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_009225
UniProt登记号 P38398
运输 wet ice
Gene Information human ... BRCA1(672)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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