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位置: 首页> 品牌> Sigma-Aldrich> Anti-A2B5 Antibody, clone A2B5-105

Anti-A2B5 Antibody, clone A2B5-105

品牌
Sigma-Aldrich
货号
MAB312
规格纯度
clone A2B5-105, Chemicon?, from mouse
价格
6022.61 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-A2B5 Antibody, clone A2B5-105
Neuron Cell Surface Antigen
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

特异性

Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).

免疫原

Embryonic chicken retinal cells

应用

Research Sub Category
Neuronal & Glial Markers
Detect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH.
Research Category
Neuroscience
Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.

Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).

May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.

May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.

Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.

Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)

Complement-mediated cytotoxicity (Eisenbarth et al., 1979)

Optimal working dilutions must be determined by end user.

外形

Format: Purified
Ammonium Sulfate Precipitation
Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Type II astrocytes, human neural progenitors

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 A2B5-105, monoclonal
species reactivity (predicted by homology) mammals
manufacturer/tradename Chemicon?
technique(s) flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
同位素/亚型 IgM
运输 wet ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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