Osteoprotegerin (OPG) human

半岛bd体育手机客户端 说明

一般描述

Osteoprotegerin (OPG) is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor κ-B ligand). The gene is mapped to human chromosome 8q24. The primary structure of OPG protein consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. It is secreted both as a monomeric and a dimeric protein. Recombinant human OPG is a soluble 20kDa protein containing 174 amino acid residues.

应用

Osteoprotegerin (OPG) human has been used as a supplement in human cell culture during cytokine production.

生化/生理作用

Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders. It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma.

序列

METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK

外形

Lyophilized from 10 mM Tris pH, 7.5 + 75 mM NaCl.

重悬

Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 7.5 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

生物来源	human
重组	expressed inE. coli
测定	≥98% (HPLC) ≥98% (SDS-PAGE)
形式	lyophilized
分子量	20.0 kDa
包装	pkg of 50 μg
technique(s)	cell culture | mammalian: suitable
杂质	<0.1 EU/μg endotoxin, tested
颜色	white to off-white
UniProt登记号	O00300
运输	wet ice
储存温度	?20℃
Gene Information	human ... TNFRSF11B(4982)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable