Granulocyte-Macrophage Colony-Stimulating Factor human

半岛bd体育手机客户端 说明

一般描述

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. Recombinant human GM-CSF is a 14.6kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

应用

Granulocyte-Macrophage Colony-Stimulating Factor human has been used in the medium for plating of the cells. It has been used in culture medium for the differentiation of human peripheral blood mononuclear cells.

生化/生理作用

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration and enhances the functional activity of the mature end-cells.

序列

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

外形

Lyophilized from 10 mM Sodium Citrate, pH 3.5.

重悬

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20℃ to -80℃.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
重组	expressed inE. coli
测定	≥98% (SDS-PAGE)
形式	lyophilized
效能	≤0.1 ng/mL
分子量	14.6 kDa
包装	pkg of 20 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	cell culture | mammalian: suitable
杂质	≤1 EU/μg endotoxin, tested
颜色	white to off-white
UniProt登记号	P04141
运输	wet ice
储存温度	?20℃
Gene Information	human ... CSF2(1437)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable