半岛bd体育手机客户端 说明
一般描述
MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
特异性
Recognizes human MDM2.
免疫原
Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.
应用
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
外形
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide
Format: Purified
分析说明
Control
POSITIVE CONTROL: Breast carcinoma
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | SMP14, monoclonal |
species reactivity | rat, human, mouse |
manufacturer/tradename | Chemicon? |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_002392.2 NM_006878.2 NM_006879.2 NM_006881.2 NM_006882.2 |
UniProt登记号 | Q00987 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich