Anti-c-Fos Antibody

半岛bd体育手机客户端 说明

一般描述

c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

特异性

This antibody recognizes the N-terminus of c-Fos.

免疫原

KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.

应用

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.
Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.

质量

Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected c-Fos in 10 ?g of PMA(TPA) treated HeLa cell lysate.

目标描述

~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)

外形

Affinity purified

分析说明

Control
PMA(TPA) treated HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat
species reactivity (predicted by homology)	bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), sheep (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_005243
UniProt登记号	P01100
运输	wet ice
Gene Information	human ... FOS(2353)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable