Rho Assay Reagent (Rhotekin RBD, agarose), 650 μg

半岛bd体育手机客户端 说明

一般描述

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
Product Source: Expressed in E. coli

质量

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

外形

650 ?g of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 ?g/mL aprotinin, 0.9 ?g/mL leupeptin, and 10% glycerol.

储存及稳定性

Stable for 6 months at -70°C from date of receipt.

其他说明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160405
NACRES	NA.84

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
manufacturer/tradename	Upstate?
technique(s)	affinity binding assay: suitable
NCBI登记号	NM_001015055.1 NM_001015056.1 NM_033046.2
UniProt登记号	Q9BST9
运输	dry ice
Gene Information	mouse ... RTKN(20166)

安全信息

危险声明	H412
预防措施声明	P273 - P501
危险分类	Aquatic Chronic 3
储存分类代码	10 - Combustible liquids
WGK	WGK 3