Rho Activation Assay Kit

半岛bd体育手机客户端 说明

一般描述

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.

应用

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

包装

Kit capacity: 30 assays

组分

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)

质量

Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32161000

半岛bd体育手机客户端 性质

质量水平	100
manufacturer/tradename	Upstate?
technique(s)	activity assay: suitable affinity binding assay: suitable
NCBI登记号	NM_001664.2
UniProt登记号	P08134 P61586 P62745
detection method	chemiluminescent colorimetric fluorometric
运输	dry ice
Gene Information	human ... RHOA(387)

安全信息

象形图
警示用语：	Danger
危险声明	H318 - H410
预防措施声明	P273 - P280 - P305 + P351 + P338 - P391 - P501
危险分类	Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3