半岛bd体育手机客户端 说明
一般描述
Intended Use
The IHC Select
General Purpose Reagents
Principle of Procedure:
The IHC Select
Summary and Explanation:
Immunohistochemical techniques have been in use for the recognition of cells in tissue sections for at least 50 years. The first method reported use of fluorescent labels in 19411. In 1974, reports of using enzymatic labels, such as horseradish peroxidase (HRP) in routine H & E paraffin sections appeared2,3. The methods have since become the ′standard of care′ in surgical pathology when classic methods alone fail to yield a definitive diagnosis4-9.
Early techniques were based on the peroxidase-anti-peroxidase (PAP) reaction, while improvements exploited the strong affinity of avidin for biotin10. This technique uses HRP-labeled streptavidin. The secondary antibody is conjugated with biotin and the Strepavidin-HRP complex reacts with the biotin on the secondary antibodies. The resulting biotin-avidin-HRP complex can react with the primary antibody bound to the specific epitope of the target antigen. The HRP enzymes of the streptavidin complex then catalyze the substrate/chromogen reaction to form a coloured reaction product (brown to black when using DAB as the chromogen) at the antigen site. A biological stain (counterstain) is then used to visualize the tissue structure.
联系
Replaces: 2240
储存及稳定性
When stored at 2 - 8°C, the IHC Select
Precautions:
· Pooling, substitution or alteration of any reagent may cause erroneous results.
* Do not mix reagents from different lots.
* Do not allow the slides to dry at any time during the staining procedure.
* Handle all specimens and materials coming in contact with them as potentially infectious materials. Disinfect with 0.05% sodium hypochlorite.
* Do not mouth pipette reagents. If ingested, seek professional assistance or contact a Poison Control Centre immediately.
· Diaminobenzidine (DAB) may be carcinogenic. This solution may cause irritation upon skin contact. Avoid inhalation, ingestion, or skin contact and wear gloves when handling DAB. If skin contact occurs, flush affected area with water. Spills may be ′neutralized′ with bleach25,26. Dispose of according to local regulations.
· Inhalation or ingestion of xylene or the highly allergenic fixative formaldehyde is harmful. These materials should be handled with gloves. Prepare in a fume hood. If skin or eye contact occurs, wash thoroughly with copious amounts of water.
· Wear disposable gloves when handling any human material and while performing the staining procedure.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 42029053 |
NACRES | NB.22 |
半岛bd体育手机客户端 性质
species reactivity | mouse, rabbit |
manufacturer/tradename | Chemicon? IHC Select |
technique(s) | immunohistochemistry: suitable (paraffin) |
detection method | colorimetric |
运输 | wet ice |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H315 - H317 - H319 - H411 |
预防措施声明 | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
危险分类 | Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
Sigma-Aldrich