H2A.X Phosphorylation Assay Kit (Flow Cytometry)

半岛bd体育手机客户端 说明

一般描述

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X.

The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.

应用

Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

包装

100 assays

组分

Anti-phospho H2A.X, FITC conjugate

Normal Mouse IgG, FITC conjugate (Cat.# 12-487)

16X Fixation Solution

10X Permeabilization Solution

10X Wash Solution

质量

phosphorylation of Histone H2A.X

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32161000
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	100
manufacturer/tradename	Upstate?
technique(s)	activity assay: suitable flow cytometry: suitable
NCBI登记号	NM_002105.2
UniProt登记号	P16104
detection method	fluorometric
运输	dry ice

安全信息

象形图
警示用语：	Danger
危险声明	H225 - H301 + H311 - H314 - H317 - H330 - H335 - H341 - H350 - H370
预防措施声明	P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类	Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
靶器官	Respiratory system
储存分类代码	3 - Flammable liquids
WGK	WGK 3