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位置: 首页> 品牌> Sigma-Aldrich> ChIPAb+ LEF1-ChIP Validated Antibody and Primer Set

ChIPAb+ LEF1-ChIP Validated Antibody and Primer Set

品牌
Sigma-Aldrich
货号
17-604
包装型号
规格纯度
from mouse
价格
6993.57 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
ChIPAb+ LEF1-ChIP Validated Antibody and Primer Set
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.

特异性

Recognizes LEF1 (lymphoid enhancer-binding factor 1).

免疫原

The antibody is made against amino acid residues 1-85 of human LEF1.

应用

Use ChIPAb+ LEF1 Mouse Monoclonal Antibody validated in WB, ChIP for the detection of LEF1.
Research Sub Category
Chromatin Biology

Transcription Factors
Western Blot Analysis:
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 mg/mL). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Research Category
Epigenetics & Nuclear Function

包装

25 assays per kit. ~4μg per chromatin immunoprecipitation.

质量

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 3x106HT29 cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Mouse Normal IgG or mouse Anti-LEF1 and the Magna ChIP?G kit (Cat.# 17-611).
Successful enrichment of LEF1 associated DNA fragments was verified by qPCR using ChIP Primers c-myc flanking the human c-myc promoter that contains LEF1 binding sites (Please see figures).
Please refer to the EZ-Magna ChIP G (Cat.# 17-409) or EZ-ChIP (Cat.# 17-371) kit protocols for experimental details.

目标描述

55-57 kDa

外形

Format: Purified
Anti-LEF1 (mouse IgG1). 1 vial containing 100 mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100 mL of phosphate buffered saline with sodium azide. Store at-20°C. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4.

Normal Mouse IgG. One vial containing 125 μg of normal mouse IgG in 125 μL volume. Store at -20°C.

ChIP primers c-myc. One vial containing 75 mL of 5 μM of each control primer specific for human c-myc promoter. Store at -20°C.
FOR: CCC AAA AAA AGG CAC GGA A
REV: TAT TGG AAA TGC GGT CAT GC

储存及稳定性

1 year at -20°C from date of shipment

分析说明

Control
Includes negative control mouse IgG antibody and control primers specific for human c-myc promoter.

法律信息

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
克隆 monoclonal
species reactivity human, mouse
manufacturer/tradename ChIPAb+
Upstate?
technique(s) ChIP: suitable
western blot: suitable
同位素/亚型 IgG1
NCBI登记号 NM_016269.2
UniProt登记号 Q9UJU2
运输 dry ice
Gene Information human ... LEF1(51176)

安全信息

储存分类代码 10 - Combustible liquids

Sigma-Aldrich

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