您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> ChIPAb+ Acetyl-Histone H4-ChIP Validated Antibody and Primer Set

ChIPAb+ Acetyl-Histone H4-ChIP Validated Antibody and Primer Set

品牌
Sigma-Aldrich
货号
17-630
规格纯度
serum, from rabbit
价格
6993.57 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
ChIPAb+ Acetyl-Histone H4-ChIP Validated Antibody and Primer Set
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H4 set includes purified rabbit polyclonal antiserum and the normal rabbit IgG antiserum, which can be used to demonstrate that the acetyl-histone H4 antibody is capable of precipitating acetyl-histone H4 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.

特异性

Recognizes acetylated histone H4 of approximately 10 kDa. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly crossreacts with acetylated histone H2B from HeLa cells.
May cross-react with other acetylated proteins.
Predicted broad cross-reactivity among eukaryotes based upon sequence similarity.

免疫原

The acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4.

应用

This ChIPAb+ Acetyl-Histone H4 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Western Blot Analysis:
Acidextracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl Histone H4 (1:2000).
Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemi-luminescence detection system. (Please see figures).

包装

25 assays per set. ~4 μL per chromatin immunoprecipitation

质量

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated HeLa cells (1 X 106cell equivalents) was subjected to chromatin immunoprecipitation using 4 μL of either a normal rabbit antiserum or Anti-Acetyl Histone
H4 serum and the Magna ChIP A (Cat. # 17-610) Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter (Please see figures).
Please refer to the EZ-Magna A ChIP (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

目标描述

10 kDa

外形

Anti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100 μL of antiserum containing 0.05% sodium azide. Store at -20°C.

Normal Rabbit Serum. One vial containing 100 uL antiserum containing 0.05% sodium azide. Store at -20°C.

Control Primers. One vial containing 75 μL of 5 μM of each primer specific for for human GAPDH.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

分析说明

Control
Included negative control rabbit IgG antiserum and control primers specific for human GAPDH promoter.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 serum
克隆 polyclonal
species reactivity human, eukaryotes
manufacturer/tradename ChIPAb+
Upstate?
technique(s) ChIP: suitable
cell based assay: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable
NCBI登记号 NP_778224
UniProt登记号 P62805
运输 dry ice

安全信息

储存分类代码 10 - Combustible liquids

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map